EVALUACIÓN DEL EFECTO TERAPÉUTICO DE UN POLIFITOFÁRMACO EN UN MODELO MURINO DE INFLUENZA TIPO A

Abstract

is an acute self-limited respiratory illness, its severe form is characterized by an intense inflammatory response with lung disease, vasodilator shock and high mortality. The etiologic agent of this disease is the influenza A virus, which belongs to Orthomixoviridae family, its genome consists of several segments of RNA, a feature that makes it easily mutate encouraging and in some cases it causes epidemics or pandemics. Pharmacologically have employed various antiviral but this response were failure because their resistance or their high costs; therefore scientific community looks for other alternatives. In this study, we search the beneficial effects of formulation fito pharmaceutical, based on licorice, cinnamon, guava, propolis, spirulina and menthol, about the infection, we evaluated the clinical, histopathological and immunohistochemical prophylactic and therapeutic treatment. Extracts of different polarity were obtained from the formulation, and we studied it in a murine model of seasonal H1N1 influenza 2010, of which the essential oil extract intranasally via was with beneficial effect. The therapeutic treatment showed no clinically significant differences, however, animals were administered prophylactically with the essential oil showed a less severe clinical illness, with no evidence of pulmonary parenchymal consolidation with an inflammatory process limited, immunohistochemical analysis showed that the decrease in the inflammatory process correlates with the decrease in NF-kB translocation to the nucleus. With the results of this thesis we can conclude that the essential oil intranasal via was shown to have anti-inflammatory effect, while extracts of ethyl acetate, hexane and water showed no such effect.