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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | MARTÍNEZ AYALA, ALMA LETICIA | - |
dc.date.accessioned | 2013-01-30T14:31:44Z | - |
dc.date.available | 2013-01-30T14:31:44Z | - |
dc.date.issued | 2013-01-30 | - |
dc.identifier.uri | http://www.repositoriodigital.ipn.mx/handle/123456789/12358 | - |
dc.description | Lima Bean (Phaseolus lunatus) Protein Hydrolysates with ACE-I Inhibitory Activity | es |
dc.description.abstract | Several protein sources can be used to produce bioactive peptides with angiotensin I-converting enzyme (ACE) inhibit- tory activity. Protein concentrates from ungerminated and germinated lima bean Phaseolus lunatus seed flours were hydrolyzed with Alcalase 2.4 L or pepsin-pancreatin sequential hydrolysis, and ACE inhibitory activity measured in the different hydrolysis treatments. Protein hydrolysate production was analyzed with a 23 factorial design with four repli- cates of the central treatment. Evaluated factors were protein concentrate source (ungerminated seeds, PC1; germinated seeds, PC2), enzyme/substrate ratio E/S (1/50 or 1/10) and hydrolysis time (0.5 or 2.0 h for Alcalase; 1 or 3 h for pep- sin-pancreatin). Degree of hydrolysis (DH) was high for the Alcalase hydrolysates (24.12% - 58.94%), but the pep- sin-pancreatin hydrolysates exhibited the highest ACE inhibitory activity (IC50 = 0.250 - 0.692 mg/mL). Under the tested conditions, the hydrolysates with the highest ACE inhibitory activity were produced with sequential pep- sin-pancreatin using either PC1 at 1 h hydrolysis time and a 1/10 E/S ratio or PC2 at 1 h hydrolysis time and a 1/50 E/S ratio. Lima bean protein hydrolysates prepared with Alcalase or pepsin-pancreatin are a potential ingredient in the pro- duction of physiologically functional foods with antihypertensive activity. | es |
dc.description.sponsorship | Instituto Politécnico Nacional CIBA-Tlaxcala | es |
dc.subject | Lima Bean | es |
dc.subject | Degree of Hydrolysis | es |
dc.subject | ACE Inhibition | es |
dc.title | Lima Bean (Phaseolus lunatus) Protein Hydrolysates with ACE-I Inhibitory Activity | es |
dc.type | Article | es |
dc.description.especialidad | Medico-Biológicas | es |
dc.description.tipo | es | |
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Art_Food and Nutrition Sciences 2012.pdf | 80.59 kB | Adobe PDF | Visualizar/Abrir |
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