“Factibilidad de detección de Trichinella spiralis en carne por clasificación de analogía espectral (SIMCA)”

Abstract

Abstract 27 The aim of this work was to study the feasibility of detection of Trichinella spiralis in 28 swine meat using Middle Infrared Spectroscopy Fourier Transform with Attenuated Total 29 Reflectance (ATR) and Soft Independent Modeling of Class Analogy (MID-FTIR-ATR-30 SIMCA). Five male Pigs were orally infected at different larvae concentrations (13000, 31 6500, 3500, 1625, 812 larvae/pig) and after 24 weeks the animals were euthanized. Five 32 types of muscles were studied (leg, loin, rib, masseter, and diaphragm). Results showed that 33 MID-FTIR-ATR-SIMCA was useful to determine the presence of T. spiralis in the 34 samples, as the interclass distance between infected and non infected muscles varied from 35 13.5 to 36.8. This technique was also useful to discriminate among pig muscles, where 36 masseter showed the largest interclass distance, while rib presented the smallest one. In all 37 cases the recognition and rejection rates was 100%, which means that the methodology is 38 capable of accurately separate T. spiralis infected from non infected swine meat.