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Campo DC | Valor | Lengua/Idioma |
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dc.creator | FIGUEROA ESPI, V. | - |
dc.creator | ALVAREZ PANEQUE, A. | - |
dc.creator | TORRENS, M. | - |
dc.creator | OTERO GONZALEZ, A.J. | - |
dc.creator | REGUERA, E. | - |
dc.date | 2012-03-23T00:24:01Z | - |
dc.date | 2012-03-23T00:24:01Z | - |
dc.date | 2011-08-11 | - |
dc.date.accessioned | 2013-01-16T09:52:41Z | - |
dc.date.available | 2013-01-16T09:52:41Z | - |
dc.date.issued | 2013-01-16 | - |
dc.identifier | Colloids and Surfaces A: Physicochem. Eng. Aspects 387 (2011) 118– 124 | - |
dc.identifier | http://hdl.handle.net/123456789/284 | - |
dc.identifier.uri | http://www.repositoriodigital.ipn.mx/handle/123456789/10779 | - |
dc.description | In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation. © 2011 Elsevier B.V. All rights reserved. | - |
dc.language | en_US | - |
dc.publisher | Colloids and Surfaces A: Physicochemical and Engineering Aspects | - |
dc.subject | Nanoparticles bioconjugate | - |
dc.subject | Nanoparticles immunoassay | - |
dc.subject | Sticholysin | - |
dc.subject | Protein separation | - |
dc.title | Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications | - |
dc.type | Article | - |
Aparece en las colecciones: | Doctorado |
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Fichero | Descripción | Tamaño | Formato | |
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Conjugation of manganese ferrite nanoparticles to an anti Sticholysin.pdf | 187.01 kB | Adobe PDF | Visualizar/Abrir |
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