Expresión proteica y regulación por estradiol y progesterona de Wnt-7a y Wnt-5a en tres regiones de útero con miomatosis

Abstract

Introduction: The uterine fibroid tumor is most common female genital tract disease and is the most common cause of gynecologic pelvic surgery. It's a classic gynecologic pathology in which almost everything is apparently well known and yet many aspects remain to be clarified from the viewpoints etiological, pathogenic and pathological, their association with fertility problems or other pathologies and therapeutic possibilities. Factors that promote the onset of fibroids and regulate their growth remain poorly understood. Have been implicated the Wnt-CPA-beta-catenin pathway as a mediator of cell proliferation in some cell lines. One of the proteins involved in the Wnt pathway is glycogen synthase kinase (GSK3). The binding of Wnt to its receptors initiates a series of events that inhibits the activity of GSK3 and prevents degradation of β-catenin. In the absence of Wnt signal, GSK3 phosphorylates and degrade β-catenin. Material and methods: An observational, analytical, prospective, and cross was performed, examining the protein expression and gene expression of Wnt-7a and Wnt-5a in tissue from three regions of the uterus with fibroids (myoma, subtumoral tissue and healthy myometrium) using techniques Western blot, immunohistochemistry and polymerase chain reaction (PCR). Results and conclusions: Wnt-7a and Wnt-5a have protein expression and a gene expression higher in fibroid tissue than in the tissues of subtumoral region and healthy myometrium. The protein expression of Wnt-7a and Wnt-5a was higher in the proliferative phase than secretory phase of the menstrual cycle. Gene expression of Wnt-7a was higher in the secretory phase of the cycle. These observations may be due to the involvement of steroid hormones in the Wnt signaling pathways, specifically in the canonical Wnt pathway, mediated by Wnt-7a, and Wnt / Ca +2 "noncanonical" mediated by Wnt-5a.