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Título : | Induction of triploidy in spotted sand bass (Paralabrax maculatofasciatus Steindachner, 1868) by cold shock |
Autor : | Alcántara-Vázquez, Juan Pablo Dumas, Silvie Puente-Carreón, Eleonora Pliego-Cortés, Hugo Skyol Peña Martínez, Renato |
Palabras clave : | Spotted sand bass cold shock triploidy |
Fecha de publicación : | 2008 |
Editorial : | Aquaculture Research |
Resumen : | Conditions for the induction of triploidy with cold shock of fertilized eggs of the spotted sand bass Paralabrax maculatofasciatus (Steindachner) were investigated. Different temperatures (12, 8 and 4 °C), timing of cold shock application (5, 10 and 15min after fertilization) and duration of the shock (5,10,15 and 20min) were tested. Triploidy was determined using flow cytometry at 12 h after larvae hatched. Triploids were produced only when the cold shock treatment was applied 5 min after fertilization. No signifiant difference was observed in the percentage of triploidy betweentemperature and the shock duration. At 8 and 4 °C, 100% triploidy was obtained at different durations of cold shock. Survivalwas significantly lower at12 or 4 °C thanat 8 °C. No significant difference was observed for shock duration at the temperature of 8 or 12 °C; however, at 4 °C, survival was signi¢cantly lower at longer durations. We recommend induction of triploidy by applying cold shock at 8 °C for a duration of 15-20 min starting at 5 min after fertilization, in the spotted sand bass |
URI : | http://www.repositoriodigital.ipn.mx/handle/123456789/12996 |
ISSN : | 1355-557X |
Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Alcantara-Vazquez_Juan_Pablo_2008_Aquaculture_Research_35.pdf | 54.67 kB | Adobe PDF | Visualizar/Abrir |
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