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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Trejo Tapia, Gabriela | - |
dc.date.accessioned | 2013-03-05T18:42:44Z | - |
dc.date.available | 2013-03-05T18:42:44Z | - |
dc.date.issued | 2013-03-05 | - |
dc.identifier.uri | http://www.repositoriodigital.ipn.mx/handle/123456789/14297 | - |
dc.description | http://link.springer.com/article/10.1007%2Fs11627-009-9250-6 | es |
dc.description.abstract | Tecoma stans is a tropical plant from the Americas. Antioxidant activity and both phenolic compound and flavonoid total content were determined for callus tissue of T. stans cultured in either a set photoperiod or in darkness. Callus lines from three explant types (hypocotyls, stem, and leaf) were established on B5 culture medium supplemented with 0.5 μM 2,4-D and 5.0 μM kinetin. While leaf-derived callus grew slower under a 16-h photoperiod (specific growth rate, μ = 0.179 d−1, t D = 3.9 d) than in darkness (μ = 0.236 d−1, t D = 2.9 d), it accumulated the highest amount (p < 0.05) of both phenolics (86.6 ± 0.01 mg gallic acid equivalents/g) and flavonoids (339.6 ± 0.06 mg catechin equivalents/g). Similarly, antioxidant activity was significantly higher (p < 0.05) when callus was cultured in period light than when grown in extended darkness. Antioxidant activity measured with a 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS)-based assay was 350.5 ± 15.8 mmol Trolox/g extract for callus cultured under a defined photoperiod compared to 129.1 ± 7.5 mmol Trolox/g extract from callus cultured in darkness. Content of phenolic compounds and flavonoids was in agreement with a better antioxidant power (EC50 = 450 μg extract/mg 1,1-diphenyl-2-picrylhydrazyl) and antiradical efficiency. Results of the present study show that calli of T. stans are a source of compounds with antioxidant activity that is favored by culture under a set photoperiod. | es |
dc.language.iso | en | es |
dc.title | COMPARISON OF METABOLITE LEVELS IN CALLUS OF TECOMA STANS (L.) JUSS. EX KUNTH. CULTURED IN PHOTOPERIOD AND DARKNESS. | es |
dc.type | Article | es |
dc.description.tipo | es | |
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