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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Escobedo Bonilla, César Marcial | - |
dc.contributor.author | Mejía Ruíz, Claudio Humberto | - |
dc.contributor.author | Vega Peña, Sergio | - |
dc.contributor.author | Alvarez Ruiz, Píndaro | - |
dc.date.accessioned | 2012-09-28T17:04:45Z | - |
dc.date.available | 2012-09-28T17:04:45Z | - |
dc.date.issued | 2011-05 | - |
dc.identifier.uri | http://www.repositoriodigital.ipn.mx/handle/123456789/7078 | - |
dc.description.abstract | Abstract The antiviral effect of vp28 or vp26 double-stranded (ds) RNA upon single or consecutive white spot syndrome virus (WSSV) intramuscular challenges with a high infectious dose was evaluated. The vp28 dsRNA showed the highest protection both in single (LT50 = 145 h at 10 d and 98 h at 20 d post treatment [dpt]) or consecutive (LT50 = 765 h) WSSV challenges compared to vp26 dsRNA (LT50 = 126 h at 10 d and 57 h at 20 dpt vs. consecutive challenge LT50 = 751 h). Single WSSV challenges showed that animals treated with vp28 or vp26 dsRNA gradually lost the antiviral effect as virus challenge occurred at 10 dpt (cumulative mortality 63% vs. 80%, respectively) or 20 dpt (87% vs. 100%, respectively). In contrast, animals treated with vp28 or vp26 dsRNA and consecutively challenged with WSSV showed and extended lower susceptibility to WSSV. All dead animals were WSSV-positive by one-step PCR, whereas all surviving shrimp from single or continuous challenges were WSSV-negative as determined by reverse transcription (RT)-PCR. In conclusion, shrimp treated with a single administration of vp28 or vp26 dsRNA and consecutively challenged with WSSV showed a stronger and longer antiviral response than shrimp exposed once to WSSV at 10 or 20 dpt. | es |
dc.language.iso | en | es |
dc.publisher | http://www.sciencedirect.com | es |
dc.subject | dsRNA | es |
dc.subject | Inhibition of virus replication | es |
dc.subject | Extended susceptibility reduction | es |
dc.subject | Consecutive WSSV inoculations | es |
dc.subject | Litopenaeus vannamei | es |
dc.title | Double-stranded RNA against white spot syndrome virus (WSSV) vp28 or vp26 reduced susceptibility of Litopenaeus vannamei to WSSV, and survivors exhibited decreased susceptibility in subsequent re-infections | es |
dc.type | Article | es |
dc.description.especialidad | Investigación | es |
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Double-stranded RNA against white spot.pdf | 80.31 kB | Adobe PDF | Visualizar/Abrir |
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