Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications

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Título :	Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications
Palabras clave :	Nanoparticles bioconjugate Nanoparticles immunoassay Sticholysin Protein separation
Fecha de publicación :	16-ene-2013
Editorial :	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Descripción :	In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation. © 2011 Elsevier B.V. All rights reserved.
URI :	http://www.repositoriodigital.ipn.mx/handle/123456789/10779
Otros identificadores :	Colloids and Surfaces A: Physicochem. Eng. Aspects 387 (2011) 118– 124 http://hdl.handle.net/123456789/284
Aparece en las colecciones:	Doctorado

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