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Título : | OXYGEN LIMITATIONS TO GROW AZADIRACHTA INDICACELL CULTURE IN SHAKE FLASKS. |
Autor : | Sepúlveda Jiménez, Gabriela |
Fecha de publicación : | 19-feb-2013 |
Resumen : | It was evaluated the growth of Azadirachta cell suspension in different conditions of oxygen delivery in Erlenmeyer shake flask. Oxygen transfer rate (OTR, kg O2 m-3 day-1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). A. indica ells growing during 6 weeks of subculture showed that lower OTR reduced cell viability, the pH of broth medium, and Azadirachtins production. While, higher OTR induced the formation of aggregates. Using a stirred tank bioreactor, it was determined that A. indica cells had an oxygen consumption of 0.100 kg O2 kg CS-1 day-1, a higher value than other plant cell cultures. These results show that OTR generated in Erlenmeyer shake flasks is lower to oxygen uptake rate of A. indica cells and it is a limiting factor to grow this plant. |
Descripción : | http://rmiq.org/new%20page/eVol10No3.html |
URI : | http://www.repositoriodigital.ipn.mx/handle/123456789/13370 |
Aparece en las colecciones: | Publicaciones |
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Articulo 29-2011.pdf | 127.05 kB | Adobe PDF | Visualizar/Abrir |
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