ORGANOGÉNESIS in vitro DE ARÁNDANO Vaccinium corymbosum L.

Abstract

To manage one to establish a protocol of regeneration of the cranberry, there were in use plants Vaccinium corymbosum L. of the variety Biloxi cultivated in field and in greenhouse. The tissues submerged in Benlate's solution and surrendered to five concentrations of hipoclorite of commercial sodium to 6 % of i.a. Sections of stem were cultivated by axilar buds in a way WPM with tiamine, mio-inositol, saccharose and agar. For the induction of outbreaks three tests were effected. In the first one the explantes surrendered to Bencildenine's different concentrations. In the second test, the axilar buds were cultivated by different concentrations of cytokinins: BA, 2- isopentiladenina and Kinetine. In the third test the explants were cultivated in way WPM by different concentrations of 2iP. To determine if the regeneration of outbreaks of blueberry needs the addition of auxins in combination with cytokinins, ANA and a witness subcultivated axilar buds in the medium of culture WPM with 2iP with four levels of the auxins without hormones. For the determination of the source of carbon sections of stem were subcultivated by a axilar buds in the medium of culture WPM with 2iP by five treatments of saccharose. The mediums WPM and modified WPM were compared. The minor pollution (30 %) was obtained with hypoclorite of sodium to 30 % v/v. The number of regenerated outbreaks and the number of leaves expanded to outbreak was major (81 and 63 %, respectively) when the explantes placed of horizontal form compared with the vertical position. With 1 ó 2 mg L-1 of BA 45.46 % was obtained of proliferation and with the concentration of 2 mg L-1 of BA the length of the outbreak was major. The best cytokinins was 2iP with 2mg L-1. The concentration of 2mg L-1 of 2iP with or 5 mg L-1 of ANA it showed longer outbreaks (4 cm). The quantity of 10 g L-1 of saccharose was the ideal one for the cranberry as well as the way modified WPM.